aquatica, have been conserved throughout the evolution of angiosperms, the following discussion attempts to place regulation of KNOX1 expression in an evolutionary context. We modified Oryza sativa (rice) plant architecture by expressing an activated version of DL (DL-VP16) in a precise manner using the DG1 sequence: the resulting transgenic plant produced a midrib in the distal region of the leaf blade, where there is no midrib in wild type, and formed more upright leaves compared with the wild type. Assuming that the above mechanisms for flexible regulation of KNOX1 expression in leaf primordia, as seen in different species of the genus Lepidium and also within a single species, N. In the tomato plant, leaves are initiated in a spiral in which the youngest primordium is designated P1, the next oldest P2, and so on in a developmental. A lateral outgrowth from the apical meristem that develops into a leaf. By contrast, the regulatory region required for carpel-specific expression was not included in the DG1 sequence. leaf primordium synonyms, leaf primordium pronunciation, leaf primordium translation, English dictionary definition of leaf primordium. In addition, a sequence termed DG1, incorporating a 5' upstream region of about 7.4kb, and introns 1 and 2, was shown to be sufficient to induce DL in the presumptive midrib, and to suppress it in other regions in the leaf primordia. Next, we confined the region to a sequence of about 200bp, which corresponds to a conserved non-coding sequence (CNS) identified by phylogenetic footprinting. A major difference between these organs is that whilst all leaves and spikelets initiated will. We found that the cis region responsible for the presumptive midrib-specific expression in the leaf primordia is located in intron 2. The internal (apical) and microscopic processes determining leaf and spikelet primordia initiation seem to be co-ordinated with leaf appearance (Kirby, 1990), which in turn determines the macroscopic, external morphological stage of Haun (1973). To understand the regulatory mechanism underlying DL expression, we tried to identify cis-regulatory regions required for temporal and spatial expression of this gene. Consistent with these functions, DL is initially expressed in the central region of the leaf primordia (presumptive midrib) and in the presumptive carpel primordia in the meristem. (B) Close-up of an NPA pin visualized by low-vacuum scanning electron microscopy. Once initiated from the SAM, leaf primordia acquire distinct polarity relative to the meristem: Cells in the leaf initial are either proximal or distal to the SAM and are either adjacent to (adaxial) or away from (abaxial) the meristem. The DROOPING LEAF (DL) gene, a member of the YABBY gene family, promotes midrib formation in the leaf and carpel specification in the flower. velopment of preexisting primordia (P1 and P2, with P1 being the youngest primordium at the beginning of the experiment). Genes involved in the differentiation and development of tissues and organs are temporally and spatially regulated in plant development.
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